Gui or GUI may refer to:

• Gui, Burkina Faso, village in Banwa Province, Burkina Faso
• Gui River, a river in China
• GUI, IOC country code for Guinea
• GUI, National Rail station code for Guide Bridge railway station in England

In computer science, a graphical user interface or GUI, pronounced ("gooey") is a type of interface that allows users to interact with electronic devices through graphical icons and visual indicators such as secondary notation, as opposed to text-based interfaces, typed command labels or text navigation. GUIs were introduced in reaction to the perceived steep learning curve of command-line interfaces (CLIs), which require commands to be typed on the keyboard.

Guizhou (; postal: Kweichow) is a province of the People's Republic of China located in the southwestern part of the country. Its provincial capital city is Guiyang.