Tn10 is a
transposable element, which is a sequence of
DNA that is capable of mediating its own movement from one position in the DNA of the host organism to another. There are a number of different
transposition mechanisms in nature, but Tn
10 uses the non-replicative cut-and-paste mechanism. The
transposase protein recognizes the ends of the element and cuts it from the original
locus. The
protein-DNA complex then
diffuses away from the donor site until random collisions brings it in contact with a new target site, where it is integrated. To accomplish this reaction the 50 kDa transposase protein must break four DNA strands to free the transposon from the donor site, and perform two strand exchange reactions to integrate the element at the target site. This leaves two strands unjoined at the target site, but the host DNA repair proteins take care of this. The target site selection is essentially random, but there is a preference for the sequence 5'-GCTNAGC-3'. The 6-9 base pairs that flank the sequence also influence selection of the insertion site.