The Tambopata River is a river in southeastern Peru and northwestern Bolivia. Most is in the Madre de Dios and Puno regions in Peru, but the upper parts of the river forms the border between Peru and Bolivia, and its origin is in La Paz department in Bolivia. The Tambopata River is a tributary of the Madre de Dios River and merges into this river at the city of Puerto Maldonado. The river flows through the Tambopata National Reserve.