Sancti Spíritus is a municipality and capital city of the province of Sancti Spíritus in central Cuba. Sancti Spíritus, Latin for "Holy Spirit". It is one of the oldest Cuban European settlements.

Sancti-Spíritus or Sancti Spiritu (Latin form of "Holy Spirit") may refer to the following locations:

• Sancti Spiritu (Argentina), first European settlement in modern Argentina
• Sancti-Spíritus, Salamanca, a municipality and village in Spain
• Sancti-Spíritus, Badajoz, Spain
• Sancti Spíritus, a municipality and city in Cuba
• Sancti Spíritus Province in Cuba
• Sancti Spiritus (cigar)