Owensboro is a home rule-class city in and the county seat of Daviess County, Kentucky, United States. It is the 4th-largest city in the state by population. It is located on U.S. Route 60 about southwest of Louisville, and is the principal city of the Owensboro metropolitan area. The city's population was 57,265 at the 2010 census, with an estimated population of 58,374 in 2014. The metropolitan population was estimated at 116,506 that same year.