The Hotham River is one of the major tributaries of the Murray River in Western Australia. It is about 160 kilometres long with its upper reaches being the Hotham River North, which begins in the Dutarning Range and joins the Hotham at its crossing of the Great Southern Highway near Popanyinning. A 15 km long southern tributary, Hotham River South begins near Cuballing and flows generally northerly before joining the Hotham near Yornaning.